School of Chemical Sciences and Pharmacy
Centre for Molecular and Structural Biochemistry
University of East Anglia, Norwich
Electron paramagnetic resonance (EPR/PELDOR) spin label study
of Multidrug efflux by P-glycoprotein (ref: RA520)
Applications are about to be invited for a full-time, fixed-term (3 year) post-doctoral research associate position available from 1st October 2008 based in the School of Chemical Sciences and Pharmacy (CAP) at the University of East Anglia (UEA). This joint project between UEA and Oxford University is funded by the Wellcome Trust.
The multidrug efflux pump P-glycoprotein (P-gp) is a major contributor to resistance in many forms of cancer. The aim of this project is to provide a molecular description of drug translocation by P-gp describing topography and local microenvironments in distinct conformational intermediates of P-gp as it progresses through its translocation pathway.
Purified P-gp protein isoforms will be provided by our collaborators in Oxford and labelled with paramagnetic probes to enable both pulsed and multi-frequency EPR/PELDOR studies. This approach will generate dynamic topographical maps of regions in P-gp involved in the process of bioenergetic coupling. The project will be carried out within the framework of the Centre for Molecular and Structural Biochemistry
The successful applicant must have a PhD and have worked in a relevant area of
biological EPR spectroscopy. Some experience of the principal pulsed EPR methodologies that will be employed during the project is beneficial. Training will be given where required.
Informal enquiries can be made to Dr Fraser MacMillan (fraser.macmillan@uea.ac.uk).
Information about the School of Chemical Sciences and Pharmacy is available at
http://www.uea.ac.uk/cap/.
Salary will be on the Grade 7/Academic 2 (RAIA) scale, range £28,290 to £33,780
(pay award pending)
Closing date: early October and interviews will follow at the end of the month.
Further particulars and an application form will become available from the
University's web page at: http://www.uea.ac.uk/hr/jobs; or by email, on
hr@uea.ac.uk ; or by calling the answerphone on 01603 593493 ; or by mail to the
Human Resources Office, University of East Anglia, Norwich NR4 7TJ, UK.
