Montag, 14. Juli 2008

Postdoctoral Position in Biomolecular NMR

Postdoctoral Position in Biomolecular NMR Spectroscopy at the Institute of Structural Biology in Grenoble, France.

A two year postdoctoral position is available to characterize the structures of viral RNAs and their complexes with human proteins using modern high-field NMR technologies. The project, to be started by the end of 2008, offers exciting opportunities to study how the Hepatitis C virus exploits host cell machineries to replicate and to use the latest, state of the art techniques in cross-correlated and liquid crystal NMR spectroscopy of nucleic acid complexes.

The laboratory hosts three NMR spectrometers equipped with cryogenic probes (two 600 MHz and one 800 MHz) and has access to the nearby 900 MHz and the first 1 GHz NMR spectrometers (end 2008) at the European high field NMR centre in Lyon. Applicants will also have access to a fully equipped wet lab for the preparation of isotopically labeled NMR samples. More information can be found on the Institute web site:

Grenoble is a pleasant city situated in the heart of the French Alps and houses one of Europe’s most vibrant structural biology communities comprising the European Synchrotron Radiation Facility (ESRF), the Laue Langevin Institute (ILL), the Structural Biology Institute (IBS) and the EMBL Grenoble Out-Station.

Previous experience in modern heteronuclear NMR spectroscopy and structure determination of biomacromolecules is required. Experience in studying RNA by NMR spectroscopy is a plus, but we will consider any motivated candidate with a good biomolecular NMR background. A competitive salary (based on previous research experience) is offered with health insurance, retirement plan and language training allowance. Interested candidates should forward a curriculum vitae, list of publication and electronic and postal addresses of potential referees to Application deadline is September 15th.

Key publications of the team in the area :

- Van Melckebeke, V., Devany, M., Di Primo, C., Beaurain, F.,. Toulmé, J.J., Bryce, D. & Boisbouvier* J. “Structural basis for stability of the HIV Trans Activation Response RNA in complex with its high affinity SELEX aptamer revealed by liquid crystal NMR“. Proc. Natl. Acad. Sci. USA, 105, 9210-9215 (2008).

- Sounier, R. Blanchard, L. Wu, Z. & Boisbouvier* J. “High-Accuracy Distance Measurement Between Remote Methyls in Specifically Protonated Proteins” J. Am. Chem. Soc 129, pp 472-473 (2007).

- Boisbouvier, J., Bryce, D., O'Neil-Cabello, E., Nikonowicz, E. & Bax* A. “Resolution optimized NMR measurement of 1DCH, 1DCC and 2DCH residual dipolar couplings in nucleic acids bases.” J. Biomol. NMR, 30, pp287-301 (2004).

- Miclet, E., Williams, D., Clore, M., Bryce, D., Boisbouvier*, J. & Bax* A. “Relaxation Optimized NMR Spectroscopy of Methylene Groups in Protein and Nucleic Acids”. J. Am. Chem. Soc 126, pp 10560-10570 (2004).

- Boisbouvier, J. , Delaglio, F. & Bax* A. “Direct observation of dipolar couplings between distant protons in weakly aligned nucleic acids” Proc. Natl. Acad. Sci. USA, 100, 11333-11338 (2003).
- Boisbouvier, J., Brutscher, B., Pardi, A., Marion, D Simorre*, J.-P. “NMR determination of sugar puckers in nucleic acids from CSA-dipolar cross-correlated relaxation“ J. Am. Chem. Soc., 122, pp 6779-6780 (2000). Postdoctoral Position in Biomolecular NMR Spectroscopy at the Institute of Structural Biology in Grenoble, France.